dss_2024

Lab Introduction

Who I am

Scientist

My name is Connor French (call me Connor) and I’m a sixth year PhD candidate in Biology- Ecology, Evolution, and Behavior! I’m advised by Dr. Mike Hickerson and unofficially co-advised by Dr. Ana Carnaval. Outside of my research I enjoy spending time outside with my dog, playing music, and film photography!

Science

I study how populations, species, and communities evolve in response to changing environments. My research has mainly centered around how population sizes and connectivity change in response to large historical climate shifts across the late Pleistocene and Holocene (~129,000 years ago to the present day) and what impact this has on species and communities. Most of my research is conducted on tropical reptiles and amphibians because they are what excite me the most, but I do work on other animals and in other areas- for instance, I recently published a paper mapping the genetic diversity of insects across the globe. I consider my research important because it offers a glimpse into how species and communities made it to where they are now, it provides a baseline to predict where they may go in the future, and perhaps most critically, whether they will survive into the future.

I use a combination of field work, lab work, and computational work to answer the questions I’m interested in. Because fossils are few and far between, I sequence the DNA of lizards and frogs across their current geographic ranges to understand their pasts. DNA contains the history of an organism’s ancestry, and even can tell us the history of entire populations- how a population may have shrank or expanded in the past and whether they exchanged migrants with other populations. After I have collected the organisms in the field and sequenced their DNA in the lab, I analyze the data on a computer. I use everything from my personal laptop to a super computer at the American Museum of Natural History to run my analyses. I use a combination of statistics, machine learning, and simulations to tease apart the potential ecological and evolutionary forces driving the patterns that I observe.

A photo of a waxy monkey tree frog I sampled in the field in the Brazilian Atlantic Forest.
Typical habitat that I conduct field work in. A fragment of tropical forest on the edge of agricultural land in the Brazilian Atlantic Forest.

Data

In addition to DNA sequences, I use ecological information to inform my analyses. I aggregate records of where the lizards or frogs I study have been observed and used that to obtain ecological information from online databases. For instance, if I want to get a broad understanding of the environmental tolerances of the tree frogs I study, I take the locations where they have been observed, extract the average annual temperature and precipitation of these locations from the online database, and summarize those values using measurements like the average or spread of those values. I can compare these ecological measurements between species or populations to see how their ecological tolerances differ and how they might change over time!

Here’s an illustration of the process:

The geographic study area is indicated with the black outline, while the observed localities of two frog species are indicated with the symbols. I can download temperature and precipitation for the study area from a database and extract those values for the observed localities to get an idea of differences in environmental tolerances for the two species.

It looks like frog species A likes it a bit cooler and wetter than frog species B!

Purpose of the lab

The lab portion of the course is mainly devoted to goals (2) and (3) from the syllabus:

(2) use exploration and experiential problem-solving to become familiar with how scientists ask questions, collect data, and analyze it to further knowledge; and 3) develop core technological skills in Microsoft Excel and the statistical programming language R to kick-start their success in future science courses and labs and research endeavors

We will use real data from the scientists you meet during the lecture portion to satisfy these goals!

Learning outcomes

What you tackle during the lab will contribute to all of the learning outcomes stated in the syllabus.

 
1. Explain how undertaking biological research differs from studying biology in a classroom.
2. Understand the diverse backgrounds from which scientists come.
3. Understand research fundamentals.
4. Understand advanced functions of Microsoft Excel.
5. Manipulate data and undertake analysis in the R statistical computing language.

How a lab will typically go

Labs will have a “Data Science” section and an “Excel/R” section. The Data Science section will cover research methods, scientific techniques, and feedback on your group projects (we will go over your group projects next week!). The Excel/R section will familiarize you with the two pieces of software and cover hands-on data recording and analysis using real data from the presenters!

Each week will involve working through a research question involving data from that week’s presenter, where you will evaluate or ask your own scientific question, form hypotheses, investigate research data, visualize and analyze the data, and interpret your analyses. The lab will focus on particular Data Science and Excel/R concepts specific to that week.

Semester outline

We have a tentative schedule for what the lab will cover, but it is subject to change depending on how I feel y’all are progressing. This is the first time this class is being taught, so I will be learning from you just like you will be learning from me!

             
Week Lecture Date Lecture Day Speaker Lab Date Lab Day Topic
1 Jan 29 Mon First class Jan 31 Wed Introduction/Excel overview
2 Feb 5 Mon Susan Perkins Feb 7 Wed Experiment components/Entering data
3 Feb 12 Mon Lincoln’s Birthday Feb 14 Wed Projects/Formula basics
4 Feb 19 Mon President’s Day      
5 Feb 21 Wed Shubha Govind Feb 22 Thu (Mon schedule). Data ethics/Import & clean data
6 Feb 26 Mon David Lohman Feb 28 Wed (Mon schedule). Experimental design/Sorting data
7 Mar 4 Mon Bao Vuong Mar 6 Wed Data visualization
8 Mar 11 Mon Mark Emerson Mar 13 Wed Filtering data
9 Mar 18 Mon David Jeruzalmi Mar 20 Wed Project check-in/Tables
10 Mar 25 Mon Mike Hickerson Mar 27 Wed Descriptive statistics
11 April 1 Mon Mark Hauber Apr 3 Wed Intro to coding/R
12 Apr 8 Mon Rob Anderson April 10 Wed Functions/Data structures
13 Apr 15 Mon Jay Edelman April 17 Wed Data visualization
14 Apr 22 Mon Spring Break      
15 Apr 29 Mon Spring Break May 1 Wed Project check-in/Statistics
16 May 6 Mon Karin Block May 8 Wed Putting everything together
17 May 13 Mon Student Presentations May 15 Wed Presentations

Grading

   
The final grade will be calculated as follows:  
Attendance 20%
Lab assignments 60%
Final projects 20%

You will have 12 lab assignments, worth 5 points each. Each lab assignment will be due before the following lab section. I will accept labs one day late for 2 point deduction, but more than one day late results in a zero. You will have opportunities throughout the semester to earn extra credit.

The final project will be a group research project that culminates in a final presentation. I will give more details on the final project on week 3.

Blackboard

All lab materials will be hosted on Blackboard. Your grades will be posted there as well. I send emails to the class through Blackboard, so make sure to check your email that’s associated with Blackboard!

Excel introduction

Installation

If you do not have installed Microsoft Office 365 installed on your computer, follow City College’s instructions to install the latest version (for free).

What is Excel?

It is a spreadsheet program developed by Microsoft. Excel organizes data in columns and rows and allows you to do mathematical functions. It runs on Windows, macOS, Android and iOS.

Why use Excel?

After you have logged on through www.office.com, click on the Excel icon to enter Excel.

After entering Excel, click on the New blank workbook button to open a new workbook

Enter a name for the workbook (call it whatever you like) and hit Enter.

There is a lot to take in on the screen, but don’t get too stressed out! We are going to tackle each component one at a time.

Note, my Excel window may look slightly different than yours. The layout should be similar, but please ask questions if something doesn’t look right!

Excel has columns and rows, displaying data in a tabular format. Rows are indicated with numbers and columns are indicated with letters. Each rectangle is a cell and is associated with a column, row index. For instance, the first cell in the workbook is column A, row 1. This would be expressed as A1.

Layout

Excel is composed of two main parts- a Ribbon and a Sheet.

The Ribbon

The Ribbon provides shortcuts to Excel commands. A command is an action that allows you to make something happen. This can for example be to: insert a table, change the font size, or to change the color of a cell.

It may seem overwhelming at first, but don’t worry- you will soon be able to navigate it with ease! There are certain commands that you will use more than others and can focus your mental efforts on finding.

The Ribbon is made up by the App launcher, Tabs, Groups and Commands.

App launcher

The App launcher icon has nine dots and is called the Office 365 navigation bar. It allows you to access the different parts of the Office 365 suite, such as Word, PowerPoint and Outlook. App launcher can be used to switch seamlessly between the Office 365 applications.

Tabs

The tab is a menu with sub divisions sorted into groups. The tabs allow users to quickly navigate between options of menus which display different groups of functionality.

Groups

The groups are sets of related commands. The groups are separated by the thin vertical line break.

Commands

The commands are the buttons that you use to do actions.

The Sheet

The Sheet is a set of rows and columns. Values can be typed to cells and can be both numbers and letters:

Can you guess the index of the cell that “Hello World” was typed in?

If you’re unsure, you can click on the cell and look at the Name Box just above the A column.

Multiple sheets

You aren’t limited to a single sheet in your workbook! To add a new sheet, you can click the plus icon next to the “Sheet#” tab, where “#” is the number of the last sheet you created.

It’s often helpful to rename your sheets to something more useful. To do this, right-click (or Ctrl-click) and select “Rename” from the pop-up menu. Enter your desired name and click “OK”.

**Try it out:
**
Try adding a new sheet, then renaming the two sheets to whatever you would like!

Excel materials inspired or sourced from W3Schools. Lab materials inspired by Data Nuggets.